禽呼肠孤病毒感染对SPF鸡跗关节细胞因子mRNA转录的影响

doi:10.11843/j.issn.0366-6964.2014.09.017

Abstract

Abstract:

Avian reovirus (ARV) is a main viral pathogen of poultry，and its infection threatens broiler production due to severe arthritis and immune suppression，leading to huge economic losses.In this study，7-day-old specific pathogen free (SPF) chickens were infected with the ARV S1133 strain through footpad injection.Tarsal joints samples were collected on day 1，7，14，21，28，35 post-infection (PI) and tested for mRNA relative transcription levels of cytokines genes of IL-1β，IL-6，IL-17，IFN-γ and TNF-α by specific real-time PCR assays.Relative transcription levels of mRNA of IL-1β，IL-6，IL-17，IFN-γ and TNF-α in tarsal joint of SPF chickens were investigated.The results showed that ARV infection up-regulated the gene transcription of these cytokines in tarsal joints and the elevated transcription continued during the course of infections，the mRNA levels of IL-1β，IL-6，IL-17，IFN-γ and TNF-α genes were up-regulated (P<0.01) compared with control groups on day 21，28，35 PI.Moreover，cytokines genes transcription of IL-1β，IL-6，IL-17 and IFN-γ have the up-regulated trends.The results revealed that IL-1β，IL-6，IL-17，IFN-γ and TNF-α participate in the ARV-induced damaging processes in the tarsal joints.This study indicated that the changes of cytokines transcription in joints of chicken infected with ARV were helpful for further understanding pathogenesis of avian reovirus infections.

CLC Number:

S852.659.4

ZHANG Kun-li，XIE Zhi-xun，HUANG Li，XIE Li-ji，LIU Jia-bo，DENG Xian-wen，XIE Zhi-qin，FAN Qing，LUO Si-si. Effect of Avian Reovirus Infection on Transcription of Cytokines mRNAs in Tarsal Joint of SPF Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1505-1511.

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Effect of Avian Reovirus Infection on Transcription of Cytokines mRNAs in Tarsal Joint of SPF Chickens

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